Myophosphorylase comes in two forms: form 'a' is phosphorylated by phosphorylase kinase, form 'b' is not phosphorylated. Form 'a' is de-phosphorylated into form 'b' by the enzyme phosphoprotein phosphatase, which is activated by elevated insulin.
Both forms 'a' and 'b' of myophosphorylase have two conformational states: active (R or relaxed) and inactive (T or tense). When either form 'a' or 'b' are in the active state, then the enzyme converts glycogen into glucose-1-phosphate.
Myophosphorylase-b is allosterically activated by elevated AMP within the cell, and allosterically inactivated by elevated ATP and/or glucose-6-phosphate. Myophosphorylase-a is active, unless allosterically inactivated by elevated glucose within the cell. In this way, myophosphorylase-a is the more active of the two forms as it will continue to convert glycogen into glucose-1-phosphate even with high levels of glycogen-6-phosphate and ATP. (See Glycogen phosphorylase§Regulation).
Structure
PYGM is located on the q arm of chromosome 11 in position 13.1 and has 20 exons.[2] PYGM, the protein encoded by this gene, is a member of the glycogen phosphorylase family and is a homodimer that associates into a tetramer to form the enzymatically active phosphorylase A. It contains an AMP binding site at p. 76, two sites involved in association of subunits at p. 109 and p. 143, and a site believed to be involved in allosteric control at p. 156. Its structure consists of 24 beta strands, 43 alpha helixes, and 11 turns. PYGM also has the following modified residues: N-acetylserine at p. 2, phosphoserine at p. 15, 2014, 227, 430, 473, 514, 747, and 748, and N6-(pyridoxal phosphate)lysine at p. 681. There is a post-translational modification in which phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.[3][4][5] Alternative splicing results in multiple transcript variants.[2]
A case study suggested that a deficiency in myophosphorylase may be linked with cognitive impairment. Besides muscle, this isoform is present in astrocytes, where it plays a key role in neural energy metabolism. A 55-year-old woman with McArdle disease has expressed cognitive impairment with bilateral dysfunction of prefrontal and frontal cortex. Further studies are needed to assess the validity of this claim.[7]
Additionally, mutations in the genes for myophosphorylase along with deoxyguanosine kinase have been associated with muscle glycogenosis and mitochondrial hepatopathy. The G456A PYGM mutation and duplication in exon 6 of dGK that results in a truncated protein have been associated with phosphorylase deficiency in muscle, cytochrome c oxidase deficiency in liver, severe congenital hypotonia, hepatomegaly, and liver failure. This expands on the current understanding of McArdle disease and suggests that this combination of mutations could result in a complex disease with severe phenotypes.[8]
An autosomal dominant mutation on the PYGM gene impairs activity of myophosphorylase-a, but not myophosphorylase-b. Symptoms include adult-onset muscle weakness and muscle biopsy shows accumulation of the intermediate filament desmin in the myofibers. Unlike McArdle disease (GSD-V, myophosphorylase deficiency), this disease does not have exercise intolerance since glycogenolysis is still possible through allosteric AMP activation of myophosphorylase-b.[9]
^Mancuso M, Orsucci D, Volterrani D, Siciliano G (May 2011). "Cognitive impairment and McArdle disease: Is there a link?". Neuromuscular Disorders. 21 (5): 356–8. doi:10.1016/j.nmd.2011.02.013. PMID21382715. S2CID36805481.