In enzymology , a precorrin-8X methylmutase (EC 5.4.99.61 ) is an enzyme that catalyzes the chemical reaction
precorrin-8X
⇌ ⇌ -->
{\displaystyle \rightleftharpoons }
hydrogenobyrinate
Hence, this enzyme has one substrate , precorrin 8X , and one product , hydrogenobyrinate .
This enzyme belongs to the family of isomerases , specifically those intramolecular transferases transferring other groups. The systematic name of this enzyme class is precorrin-8X 11,12-methylmutase . Other names in common use include precorrin isomerase , hydrogenobyrinic acid-binding protein and CobH . This enzyme is part of the biosynthetic pathway to cobalamin (vitamin B12 ) in aerobic bacteria .
See also
Structural studies
As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1F2V , 1I1H , 1OU0 , 1V9C , 2AFR , and 2AFV .
References
Blanche F; Couder, M; Famechon, A; Debussche, L; Cameron, B; Crouzet, J; Blanche, F (1992). "The final step in the biosynthesis of hydrogenobyrinic acid is catalyzed by the cobH gene product with precorrin-8x as the substrate" . J. Bacteriol . 174 (3): 1043–9. doi :10.1128/JB.174.3.1043-1049.1992 . PMC 206186 . PMID 1732194 .
Roth JR, Lawrence JG, Rubenfield M, Kieffer-Higgins S, Church GM (1993). "Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium" . J. Bacteriol . 175 (11): 3303–16. doi :10.1128/jb.175.11.3303-3316.1993 . PMC 204727 . PMID 8501034 .
Stolowich NJ, Iida K, Scott AI (1992). "Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis. Identification of the 11-methyl and 20-methyl transferases of corrin biosynthesis" . FEBS Lett . 301 (1): 73–8. doi :10.1016/0014-5793(92)80213-Z . PMID 1451790 . S2CID 20198692 .
Thibaut D, Debussche L (1990). "Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid" . J. Bacteriol . 172 (10): 5980–90. doi :10.1128/JB.172.10.5980-5990.1990 . PMC 526920 . PMID 2211521 .
Activity Regulation Classification Kinetics Types