In 1901, Trustrup station became the northern terminus of the Ebeltoft Line to the town of Ebeltoft. From 1932, however, all trains on the Ebeltoft Line were continued on the tracks on the Grenaa Line from Trustrup to Grenaa. The Ebeltoft Line was closed in 1968.[4]
From 2016 to 2019, the station was temporarily closed along with the Grenaa railway line while it was being reconstructed and electrified to form part of the Aarhus light rail system, a tram-train network combining tram lines in the city of Aarhus with operation on railway lines in the surrounding countryside.[2] Since 2019, the station has been served by Line L1 of the Aarhus light rail network, operated by the multinational transportation company Keolis.[5]