Class of enzymes
Acyl-homoserine-lactone synthase (EC 2.3.1.184 ) is an enzyme with systematic name acyl-(acyl-carrier protein):S-adenosyl-L-methionine acyltranserase (lactone-forming, methylthioadenosine-releasing) .[ 1] [ 2] [ 3] [ 4] [ 5] [ 6] [ 7] [ 8] [ 9] This enzyme catalyses the following chemical reaction
acyl-[acyl-carrier protein] + S-adenosyl-L-methionine
⇌ ⇌ -->
{\displaystyle \rightleftharpoons }
[acyl-carrier protein] + S-methyl-5'-thioadenosine + N-acyl-L-homoserine lactone
Acyl-homoserine lactones (AHLs) are produced by a number of bacterial species and are used by them to regulate the expression of virulence genes in a process known as quorum-sensing .
Alternate names
acyl-homoserine lactone synthase , acyl homoserine lactone synthase , acyl-homoserinelactone synthase , acylhomoserine lactone synthase , AHL synthase , AHS , AHSL synthase , AhyI , AinS , AinS protein , autoinducer synthase , autoinducer synthesis protein rhlI , EsaI , ExpISCC1 , ExpISCC3065 , LasI , LasR , LuxI , LuxI protein , LuxM , N-acyl homoserine lactone synthase , RhlI , YspI , acyl-[acyl carrier protein]:S-adenosyl-L-methionine acyltranserase (lactone-forming, methylthioadenosine-releasing)
References
^ Schaefer AL, Val DL, Hanzelka BL, Cronan JE, Greenberg EP (September 1996). "Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein" . Proceedings of the National Academy of Sciences of the United States of America . 93 (18): 9505–9. doi :10.1073/pnas.93.18.9505 . PMC 38458 . PMID 8790360 .
^ Watson WT, Murphy FV, Gould TA, Jambeck P, Val DL, Cronan JE, Beck von Bodman S, Churchill ME (December 2001). "Crystallization and rhenium MAD phasing of the acyl-homoserinelactone synthase EsaI". Acta Crystallographica D . 57 (Pt 12): 1945–9. doi :10.1107/s0907444901014512 . PMID 11717525 .
^ Chakrabarti S, Sowdhamini R (April 2003). "Functional sites and evolutionary connections of acylhomoserine lactone synthases" . Protein Engineering . 16 (4): 271–8. doi :10.1093/proeng/gzg031 . PMID 12736370 .
^ Hanzelka BL, Parsek MR, Val DL, Dunlap PV, Cronan JE, Greenberg EP (September 1999). "Acylhomoserine lactone synthase activity of the Vibrio fischeri AinS protein" . Journal of Bacteriology . 181 (18): 5766–70. doi :10.1128/JB.181.18.5766-5770.1999 . PMC 94098 . PMID 10482519 .
^ Parsek MR, Val DL, Hanzelka BL, Cronan JE, Greenberg EP (April 1999). "Acyl homoserine-lactone quorum-sensing signal generation" . Proceedings of the National Academy of Sciences of the United States of America . 96 (8): 4360–5. doi :10.1073/pnas.96.8.4360 . PMC 16337 . PMID 10200267 .
^ Ulrich RL (October 2004). "Quorum quenching: enzymatic disruption of N-acylhomoserine lactone-mediated bacterial communication in Burkholderia thailandensis" . Applied and Environmental Microbiology . 70 (10): 6173–80. doi :10.1128/AEM.70.10.6173-6180.2004 . PMC 522112 . PMID 15466564 .
^ Gould TA, Schweizer HP, Churchill ME (August 2004). "Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI" . Molecular Microbiology . 53 (4): 1135–46. doi :10.1111/j.1365-2958.2004.04211.x . PMID 15306017 .
^ Raychaudhuri A, Jerga A, Tipton PA (March 2005). "Chemical mechanism and substrate specificity of RhlI, an acylhomoserine lactone synthase from Pseudomonas aeruginosa". Biochemistry . 44 (8): 2974–81. doi :10.1021/bi048005m . PMID 15723540 .
^ Gould TA, Herman J, Krank J, Murphy RC, Churchill ME (January 2006). "Specificity of acyl-homoserine lactone synthases examined by mass spectrometry" . Journal of Bacteriology . 188 (2): 773–83. doi :10.1128/JB.188.2.773-783.2006 . PMC 1347284 . PMID 16385066 .
External links
Activity Regulation Classification Kinetics Types